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314 bp activina r d systems 338 ac 050 xav939 stemgent  (R&D Systems)


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    R&D Systems 314 bp activina r d systems 338 ac 050 xav939 stemgent
    314 Bp Activina R D Systems 338 Ac 050 Xav939 Stemgent, supplied by R&D Systems, used in various techniques. Bioz Stars score: 97/100, based on 1145 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/activina+338+ac+r+d+systems/Recombinant+Human+BMP-4+Protein/pm35325615-254-45-47
    Average 97 stars, based on 1145 article reviews
    314 bp activina r d systems 338 ac 050 xav939 stemgent - by Bioz Stars, 2026-10
    97/100 stars

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    Article Title: A ligand/receptor trafficking clock governs self-renewal and abscission dynamics in pluripotent stem cells
    Article Snippet: The medium was changed the day after with RSC differentiation medium (N2B27 medium supplemented with 2 μM IWP2 (681671, Sigma) and 1μM PD0325901 (444968, Sigma) or EpiLC differentiation medium (N2B27 medium supplemented with 20 ng/mL ActivinA (338-AC-010/CF, R&D Systems) and 12ng/mL bFGF (3139-FB-025/CF, R&D Systems)) and cells were collected 48h after for subsequent analysis.

    Article Title: A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes
    Article Snippet: Differentiation was initiated the following day (Day 0) with the addition of RPMI-1640 supplemented with B27 without insulin, 100 ng/mL ActivinA (R&D Systems #338-AC), and 1:60 diluted growth-factor-reduced Matrigel.

    Article Title: Selective activation of FZD7 promotes mesendodermal differentiation of human pluripotent stem cells
    Article Snippet: DOI: https://doi.org/10.7554/eLife.63060 17 of 28 Continued Reagent type (species) or resource Designation Source or reference Identifiers Additional information Antibody PE anti-human CD45, mouse monoclonal Biolegend Cat# 304008 1:100 RRID:AB_314396 Antibody APC mouse IgG2a k isotype control, mouse monoclonal Biolegend Cat# 400222 1:100 Antibody PE mouse IgG1, k isotype control, mouse monoclonal Biolegend Cat# 400112 1:100 RRID:AB_2847828 Antibody Alexa-Fluor 488 Goat anti-Mouse IgG, goat polyclonal Invitrogen Cat# A11001 1:1000 RRID:AB_2534069 Recombinant protein F7L6 This paper Purified from CHO_His-F7L6-Fc, available upon request from corresponding author Recombinant protein F7L6-sc This paper Purified from CHO_His-F7L6, available upon request from corresponding author Recombinant protein Wnt3a Produced in Willert lab Willert et al., 2003 Willert, 2008 Purified from CHO_Wnt3a cells Recombinant protein RSPO1 Prof. Xi He, Harvard Medical School Purified from HEK293 _Rspo1 cells Recombinant protein FLAg FP+P-L61+3 Prof. S. Angers, Toronto University, Canada Tao et al., 2019 Recombinant protein ActivinA R and D Systems Cat# 338-AC 100 ng/mL Recombinant protein BMP4 R and D Systems Cat# 314 BP 40 ng/mL Recombinant protein SCF R and D Systems Cat# 7466-SC 40 ng/mL Recombinant protein VEGF R and D Systems Cat# 293-VE 20 ng/mL Recombinant protein FGF2 StemCell Technologies Cat#78003 10 ng/mL Commercial assay or kit SuperSignal West Dura Western Blot Substrate ThermoFisher Scientific Cat# 34075 Commercial assay or kit Pierce Coomassie (Bradford) Protein Assay Kit ThermoFisher Scientific Cat# 23200 Commercial assay or kit TRIzol Reagent ThermoFisher Scientific Cat# 15596026 Commercial assay or kit Direct-zol RNA MiniPrep Kit Zymo Research Cat# R2051 Commercial assay or kit iScript Reverse Transcription Supermix Bio-Rad Cat# 1708840 Commercial assay or kit iTaq Universal SYBR Green Supermix Bio-Rad Cat# 1725120 Chemical compound, drug Zeocin ThermoFisher Scientific R25005 1 mg/mL Chemical compound, drug Puromycin ThermoFisher Scientific A1113802 4 mg/mL Chemical compound, drug Rock inhibitor Y-27631 Tocris Cat# 1254 5 mM Chemical compound, drug GSK3 inhibitor CHIR98014 Sigma-Aldrich Cat# SML1094 250 nM Chemical compound, drug ATP Sigma-Aldrich Cat# A2383 Continued on next page Gumber, Do, et al. eLife 2020;9:e63060.

    Article Title: A comprehensive analysis of gene expression changes in a high replicate and open-source dataset of differentiating hiPSC-derived cardiomyocytes
    Article Snippet: Differentiation was initiated the following day (Day 0) with the addition of RPMI-1640 supplemented with B27 without insulin, 100 ng/mL ActivinA (R&D Systems #338-AC), and 1:60 diluted growth-factor-reduced Matrigel.



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    Journal: Cell

    Article Title: A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets

    doi: 10.1016/j.cell.2018.12.003

    Figure Lengend Snippet: KEY RESOURCES TABLE

    Article Snippet: ​ REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Rat anti C-peptide DSHB Cat#GN-ID4 Rabbit anti Chga Abcam Cat#Ab15160 Rabbit anti Cldn4 Lifespan BioSciences Cat#LS-B8382 Mouse anti E-cadherin BD Transduction Laboratories Cat#610181 Chicken anti GFP Aves Labs inc. Cat#GFP-1020 Goat anti glp2 Santa Cruz Biotechnology Cat#Sc-7781 Guinea pig anti Insulin DAKO Cat#A0564 Hamster anti Muc1 NeoMarkers Cat#HM-1630-P1 Rabbit anti Ncad Invitrogen Cat#MA5–16324 Goat anti Neurod1 R&D systems Cat#AF2746 Rabbit anti Neurogenin3 EMD Millipore Cat#Ab10535 Mouse anti Nkx6.1 DSHB Cat#F55A12 Rabbit anti proglucagon Cell Signaling Technology Cat#D16G10 Rabbit anti Slug Cell Signaling Technology Cat#95855 Rabbit anti Sox9 Abcam Cat#Ab-5535 Goat anti somatostatin Santa Cruz Biotechnology Cat#Sc-7819 Rabbit anti Synaptophysin ProteinTech Group Cat#17785–1-AP Chicken anti Vimentin Encor Biotechnologies Cat#CPCA-Vim Mouse anti Zeb1 eBioscience Cat#14–9741-82 Rabbit anti α SMA Abcam Cat#Ab5694 Chemicals, Peptides, and Recombinant Proteins ActivinA R&D Systems Cat#338-AC Rock Inhibitor Y-27632 DNSK Cat#DNSK-Kl15–02 Critical Commercial Assays ViewRNA ISH Tissue Assay Kit (2-plex) ThermoFisher Cat#QVT0012 SMARTScribe Reverse Transcriptase Clontech Cat#639537 Advantage2 Polymerase mix Clontech Cat#639202 Agencourt Rnaclean ThermoFisher Cat#NC0068576 Agencourt AMPure Beckman Coulter Cat#A63881 Direct-zol ThermoFisher Cat#50444622 Deposited Data Raw and analyzed single cell sequencing data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE121416","term_id":"121416"}} GSE121416 Raw and analyzed bulk sequencing data This paper GEO: {"type":"entrez-geo","attrs":{"text":"GSE121679","term_id":"121679"}} GSE121679 Experimental Models: Cell Lines HUES8 hESCS HSCI hES Cell line: HUES-8 Experimental Models: Organisms/Strains Mouse: Neurog3 tm1(EGFP)Khk Raised in house RRID:MMRRC_000344-UCD Mouse: CB17.Cg-Prkdc scid Lyst bg-J /Crl Charles River RRID:IMSR_CRL:250 Software and Algorithms Houdini Side Effects Software www.sidefx.com Topic modeling This paper http://groups.csail.mit.edu/cgs/geneprogram.html Monocle Trapnell lab http://cole-trapnell-lab.github.io/monocle-release/ Open in a separate window KEY RESOURCES TABLE Mapping changes in gene expression during pancreatic islet formation using scRNA-seq Islets form as budding “peninsulas,” not through aggregation of dispersed cells Islet architecture reflects the temporal order of α and β cell appearance Peninsula-like buds generated in vitro from differentiating pluripotent cells

    Techniques: Transduction, Recombinant, Reverse Transcription, Sequencing, Software